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Registros recuperados: 2.463 | |
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Altinkut,A.; Kazan,K.; Gozukirmizi,N.. |
The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013 |
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Guerra Jr.,José Carlos Vieira; Gonçalves,Lionel Segui; Jong,David De. |
Africanized honey bees are more tolerant of infestations with the mite Varroa jacobsoni than are honey bees of European origin. The capacity of these bees to detect and react to brood infested with this mite could be one of the factors determining this tolerance. We tested colonies of Africanized bees headed by queens from swarms collected in Ribeirão Preto, São Paulo State. The Italian colonies had queens imported directly from the USA, or from the Brazilian Island of Fernando de Noronha, where varroa-infested Italian colonies have been maintained, untreated, since 1984. Recently sealed worker brood cells were artificially infested by opening the cell capping, inserting live adult female mites and resealing the cells. Control cells were treated in the... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100016 |
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Gonçalves,Paulo de Souza; Bortoletto,Nelson; Cardinal,Átila Bento Beleti; Gouvêa,Lígia Regina Lima; Costa,Reginaldo Brito da; Moraes,Mário Luiz Teixeira de. |
In a field trial involving 68 rubber tree (Hevea spp.) clones calculation of genotypic correlation coefficients revealed significant age-age correlation from age 1 to 6 (immature period) for girth A and for age 7 to 12 (mature period) for girth B and for age 7 to 12 (production of latex) for yield. Rank correlation coefficients between all immature ages of girth (girth A), all ages of mature girth (girth B) and all annual rubber production (yield) were significant for the three traits, with the coefficients decreasing with increasing age. Selection of the sets of best 30, 15, 10 and 5 clones from the available 68 clones at a given age was generally accompanied by a descending order of percentage success. It was suggested: (a) to have the best 30 clones of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hevea brasiliensis; Early clonal multiplication; Genetic correlation; Rank correlation; Evaluation of clones. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500018 |
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del Solar Osses,Eduardo. |
A study was made of the spatial pattern of the progeny produced in boxes with 25 oviposition tubes, by groups of six Drosophila melanogaster females during 24, 72 and 216 h. Each female was fertilized by a male carrier of a dominant mutation in such a manner that the behavior of each female of the group as well as that of each group could be determined by a study of their progeny. The pattern of spatial distribution of the progeny was aggregated, reaching its highest value at 72 h. Gregarious behavior of individuals was defined by the number of sites among which they disseminated their progeny in a 24 h-cycle; while for groups it was estimated by the variance/mean ratio. The fertility, egg-adult development time and mortality rate were recorded.... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100006 |
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Guerra,Marcelo. |
Abstract Agmatoploidy is a type of chromosome rearrangement that involves the fragmentation of an entire chromosome complement, generating a diploid with double its original chromosome number. Agmatoploidy and other related karyotype changes, such as symploidy (the opposite change, promoted by chromosome fusion), partial agmatoploidy, polyagmatoploidy, etc., are restricted to species with holokinetic chromosomes and are assumed to play an important role in their karyotype evolution. However, a critical review of the literature shows that examples of chromosome number doubling by agmatoploidy are rare and not clearly demonstrated, while partial agmatoploidy and partial symploidy seem to be the same as ascending and descending disploidy, respectively. It is... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agmatoploidy; Symploidy; Holocentrics; Holokinetic chromosomes; Chromosome evolution. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400492 |
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Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.. |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022 |
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Augustin,Lizete; Federizzi,Luiz Carlos; Sereno,Maria Jane Cruz de Melo. |
Immature embryo-derived somaclones regenerated from genotypes UPF 12, UPF 89S080 and UFRGS 7 were analyzed for eight agronomic traits and two enzymatic systems in order to evaluate the potential of tissue cultures to induce genetic variability in oats (Avena sativa L.). Some somaclones were also analyzed cytogenetically. Agronomic traits were evaluated in the field for all somaclones in 1993 and 1994. Bi-directional variation (P < 0.05) was detected for all characteristics, and the average frequency of variation observed in 1993 in somaclones from genotypes UPF 12 and UPF 89S080 was 35.2%. Variation observed for agronomic characteristics was heritable through two generations. Isoenzymatic analysis showed variation for both enzymatic systems in four... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300025 |
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Baldoni,Aisy B.; Ramalho,Magno A. Patto; Abreu,Ângela de Fátima B.. |
Strategies were investigated for improving efficiency in the use of segregating common bean (Phaseolus vulgaris) populations using crosses between the Andean cultivar BRS-Radiante and the Mesoamerican parent cultivar Carioca-MG by developing populations with 12.5%, 25%, 50%, 75% and 87.5% of the allele frequency of one of the parents. For each of the five populations we evaluated for two traits, the number of days to the beginning of flowering and grain yield (g plot-1), in the F2:3 (sown in February 2006) and F2:4 (sown in July 2006) generation progenies using 15 x 15 lattice design experiments, with 44 progenies (n = 220 plants) plus the two parents and three controls being evaluated for each generation. In terms of variability release, the populations... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Backcross; Gene pools; Heritability; Phaseolus vulgaris; Selection gain. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500019 |
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Vallinoto,Izaura Maria Vieira Cayres; Vallinoto,Antonio Carlos Rosário; Valente,Cristina Maria Duarte; Guerreiro,João Farias. |
The allele frequency distributions of three VNTR (D1S80, APOB and D4S43) and three STR (vW1, F13A1 and DYS19) loci were investigated in two Afro-Brazilian populations from the Amazon: Curiau and Pacoval. Exact tests for population differentiation revealed significant differences in allele frequency between populations only for the D1S80 and APOB loci. A statistically significant deviation from the Hardy-Weinberg equilibrium was observed only in the D1S80 locus of the Pacoval sample. A neighbor-joining tree was constructed based on DA genetic distances of allele frequencies in four Afro-Brazilian populations from the Amazon (Pacoval, Curiau, Trombetas, and Cametá), along with those from Congo, Cameroon, Brazilian Amerindians, and Europeans. This analysis... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA polymorphisms; Afro-Brazilians; Hypervariable loci. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300003 |
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Francez,Pablo Abdon da Costa; Rodrigues,Elzemar Martins Ribeiro; Frazão,Gleycianne Furtado; Borges,Nathalia Danielly dos Reis; Santos,Sidney Emanuel Batista dos. |
The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F ST coefficients) to the present database ranged from F ST = 0.0016 between Macapá and Belém to F ST =... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allelic frequencies; Brazilian Amazon population; Interethnic admixture. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100007 |
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Gonçalves-Vidigal,Maria Celeste; Silva,Claudete Rosa da; Vidigal Filho,Pedro Soares; Gonela,Adriana; Kvitschal,Marcus Vinícius. |
The genetic resistance of Phaseolus vulgaris L. cultivar Michelite to races 8 and 64 of Colletotrichum lindemuthianum, causal agent of bean anthracnose, was characterized. Crosses were made between Michelite and Mexico 222 cultivars and the F2 population was inoculated with race 64 in order to study the inheritance of resistance to anthracnose in Michelite. The segregation of F2 population fitted in a ratio of 3R:1S, showing the presence of a dominant gene in Michelite gene conditioning resistance to race 64. Allelism tests were conducted with F2 populations derived from crosses between Michelite and AB 136, AND 277, BAT 93, Cornell 49-242, G 2333, Kaboon, Mexico 222, Michigan Dark Red Kidney (MRDK), Ouro Negro, Perry Marrow, PI 207262, TO, TU, and Widusa.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colletotrichum lindemuthianum; Gene pool; Mesoamerican resistance gene; Phaseolus vulgaris L.. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400015 |
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Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H.. |
Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034 |
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Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana. |
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025 |
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Toro,Jorge E.; Castro,Grace C.; Ojeda,Johana A.; Vergara,Ana M.. |
Genetic differentiation in the Chilean blue mussel Mytilus chilensis (Hupé 1854) was investigated based on the variation in the allozyme frequencies of Pgm, Gpi, Icd, Me, Gsr, Lap and Pep in eight samples collected along 1800 km from Arauco (VIII Region) to Punta Arenas (XII Region). Despite the large geographic separations, values of Neis unbiased genetic distance, D (0.004-0.048) and standardised genetic variation among populations, Fst (0.011-0.055) were small. The levels of gene flow (Nm = 8) found in this study prevent the effect of differentiation among populations by genetic drift. This findings indicate that its long-lived planktotrophic larvae provides this species with considerable dispersal ability throughout its range which is favoured by the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozyme; Mytilus chilensis; Population genetics; Gene flow; Chile. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100031 |
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Denden,Sabri; Khelil,Amel Haj; Knani,Jalel; Lakhdar,Ramzi; Perrin,Pascale; Lefranc,Gérard; Chibani,Jemni Ben. |
Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpha-1 antitrypsin; SERPINA1 polymorphisms; COPD; Emphysema; Lung function. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100006 |
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Luz,Julio A. da; Sans,Mónica; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins; Sonati,Maria de Fatima; Costa,Fernando Ferreira. |
Hemoglobinopathies are the most common monogenic disorders worldwide; however, they have never been systematically studied from a genetic perspective in Uruguay. In this study, we determined the frequencies of hemoglobin variants in Afro-Uruguayans. A sample of 52 healthy unrelated Afro-Uruguayans from the northern (N = 28) and southern (N = 24) regions of the country was analyzed. Eight individuals (15.4%) were heterozygous for -alpha3,7thalassemia; seven of them (29.2%) were originally from the southern region, whereas one of them (3.6%) was from the northern region; the differences between both regions were statistically significant (p = 0.016 +/-0.003). The only structural mutation detected was betaS, which is typical of African populations. Four... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemoglobinopathies; Alpha-thalassemias; HbS; Haplotypes; Afro-derived populations. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400002 |
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Registros recuperados: 2.463 | |
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